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psat1  (Proteintech)
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Proteintech psat1
Fig. 4. <t>PSAT1</t> is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.
Psat1, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fig. 4. PSAT1 is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.

Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Article Title: MicroRNA-340 Inhibits Esophageal Cancer Cell Growth and Invasion by Targeting Phosphoserine Aminotransferase 1.

doi: 10.1159/000430361

Figure Lengend Snippet: Fig. 4. PSAT1 is a direct target of miR-340. (A) The putative miR-340-binding sequence in the 3′-UTR of PSAT1 mRNA is shown. Muta tions were introduced into the PSAT1 3′-UTR sequence at the complementary site for the seed region of miR-340. (B) Luciferase reporter as says show the miR-340-me diated suppression of the ac tivity of the wild-type PSAT1 3′-UTR luciferase, but not the mutant 3′-UTR luciferase, in HEK-293 cells. (C) Ectopic ex pression of miR-340 in EC109 and EC7906 cells downregu lated the mRNA expression of PSAT1. (D) PSAT1, GSK- 3β, p-GSK-3β (Ser9), Snail, Vimentin and E-cadherin protein levels were deter mined by western blotting. *, p < 0.05; **, p < 0.01.

Article Snippet: The following antibodies were used: p-GSK-3β, GSK-3β, Snail, E-cadherin, Vimentin (Cell Signaling Technology); and PSAT1 (Proteintech Group Inc, Chicago, IL).

Techniques: Binding Assay, Sequencing, Luciferase, Mutagenesis, Expressing, Western Blot